December 12, 2010
Since there is very little information still available for the Nikon Fluophot Microscope, unless you are well versed in the use of other microscopes with the same kind of attachments, then it will be hard for you to get good quality images or views through the eyepieces. I have found that virtually every time I use the Fluophot, I must make these adjustments before using the scope. You should get the owners manual from Nikon which gives some basic information about mounting the condenser and some of the settings. This page will explain how I set up and make adjustments to my scope and condenser before actually using the scope for viewing or photography.
The following process should be done every day you use the scope. Not sure why settings can change, but they do, and it's good to check them anyway.
CENTERING THE LAMP FILAMENT:
1. Start by centering the Halogen lamp. Turn the power switch on and set the voltage meter to 7-8. Use the Dark Grey filter on the right side of the VD-Base of the scope, so you don't burn your eyes. Place a slide on the stage and focus on the specimen using the 10x objective. Open the condenser iris and Field Diaphragm to their widest settings.
2. Turn the Diffuser Lever on the lamp housing to the Out position. Turn the Focus Knob of the collector lens, [on the left side of the lamp housing], to form a sharp image of the lamp filament. This may take some adjustment of the scope focus knob and the lamp focus knob. But eventually you will get a sharp image of the filament.
3. I do not have a Lamp Centering Tool, but it's not really needed. Using the lateral and vertical centering screw on the right side of the lamp housing, adjust the image of the lamp filament up or down, right or left, to center the filament in the view. The filament is larger than the view field, so you will have to make an educated guess, but it's pretty obvious.
4. Now you are done with centering the filament. Don't forget to turn the diffuser lever back to the IN position.
CENTERING THE CONDENSER:
1. Close the Field Diaphragm.
2. Close the iris on the bottom of the condenser. Line up the 10x or 40x Objective lens. [You will have to center the condenser for each one before using it.]
3. Flip up the condenser lens so the light comes through it.
4. Raise the condenser to it's highest position. [It should not hit the bottom of the slide]
5. Now, open the Field Diaphragm until you see a hexagon light pattern. Keep it small. Focus the scope on the slide until the hexagon is sharp. I move the slide so I am looking through a clean part.
6. Use the condenser adjustment screws to center the hexagon in the field of view. Take the time to do it right.
7. Once the hexagon is centered, open the Field Diaphragm until the light pattern is just slightly bigger than the field of view. Open the condenser iris enough to increase the light amount a little bit. You might have to lower the condenser a bit to get rid of the hot spot.
8. Turn the light voltage down until the amount of light is comfortable. Pull out one of the Ocular lenses and look into the hole for the bright spot of light. Open the condenser iris until it fills about 70% of the field of view. You are now ready to view a subject on the slide.